PROTEIN
FACILITY ----------------------------------------------------------------------------
The Protein Facility
at LSU, jointly supported by the College of Basic
Sciences and the LSU Agricultural Center, is equipped
to synthesize, purify,
and characterize peptides and proteins.
The facility, under the direction of Martha Juban,
offers an economical and cost effective alternative
to commercial facilities which charge up to ten
times as much. To see the facility's online brochure,
click here.
The following instrument
systems are available in the facility:
1.
Applied
Biosystems PIONEER peptide synthesizer,capable
of Fmoc synthesis at scales from 0.05 to 2 millimoles.
Charges are based on sequence length.
2.
Waters
HPLC systems for analytical and semi-preparative
scale work, including reverse-phase C4 and C18
columns, and a photodiode array detector. Charges
are based on the quantity of crude material
processed.
3.
Dionex
AAA-Direct ion chromatography system for amino
acid analysis at the picomole level. Charges
are based on number of samples hydrolyzed.
4.
Aviv
62 DS circular dichroism spectrophotometer to
determine secondary structure in solution.Charges
are based on hours used.
5.
Beckman
Optima XL-A analytical ultracentrifuge for both
sedimentation velocity and sedimentation equilibria
experiments .Charges are based on hours used.
Peptide
synthesis , HPLC purification ,and amino acid
analysis are completed by facility personnel.
For CD and AU,the facility provides basic training
in the use of the instrument but users are expected
to conduct their own experiments.
