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LSU
Protein Facility |
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LSU
Protein Facility |
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The Protein Facility at LSU, jointly supported by the College of Basic Sciences and the LSU Agricultural Center, is equipped to synthesize, purify, and characterize peptides and proteins. Specialized instrumentation includes an Applied Biosystems Pioneer peptide synthesizer (Fmoc chemistry), analytical and preparative scale Waters HPLC systems, a Dionex AAA-Direct amino acid analyzer, an Aviv circular dichroism spectrometer, and a Beckman Optima XL-A analytical ultracentrifuge. The facility offers an economical and cost effective alternative to commercial facilities which charge up to ten times as much.
A preliminary estimate of the cost of custom peptide synthesis will be determined based on the length and complexity of the specified sequence. The basic fee will be $25 per amino acid for a 0.2 mmol scale synthesis.
The final price will be determined after receipt of the written sequence. It may be higher if an unusually large number of protected side-chain amino acids are required. This price includes an analytical chromatogram.
Crude peptide product will be purified by reverse-phase HPLC at a cost of $150 per 100 mg. The purified peptides will be supplied as lyophilized trifluoroacetate salts two to four weeks after synthesis begins. A successful synthesis of a 20-mer can be expected to yield 100-200 mg, at least 95% pure by HPLC.
Peptide identity will be verified by the Mass Spectrometry Facility in Choppin Hall, at an additional cost of $15 per sample.
Amino acid analysis is available at a cost of $75 per sample. Samples are hydrolyzed, separated on a anion exchange column and quantitated by integrated amperometry.
The Aviv 62 DS circular dichroism spectrophotometer measures spectra of peptides and proteins to determine secondary structure in solution at a cost of $3 per hour plus a fee for liquid nitrogen.
The Beckman Optima XL-A instrument may be used for sedimentation velocity and sedimentation equilibrium studies to determine molecular weights and aggregation states of peptides and proteins in solution.
Contact the facility to request a cost estimate for your specific synthetic or analytical requirement. The Director will respond to requests as quickly as possible. Weekly updates on the status of orders are provided.
Martha Juban, Facility Director
mjuban@lsu.edu
439 Choppin Hall (facility/office)
338 Choppin Hall (mailing address)
Baton Rouge, LA 70803
(225) 578-5230 578-8826 FAX
Dr. Robert Hammer, Faculty Advisor
Department of Chemistry
Louisiana State University
232 Choppin Hall
Baton Rouge, LA 70803-1804
Office: (225) 578-4025 FAX: (225) 578-3458
Email: rphammer@lsu.edu
Web: http://chemistry.lsu.edu/rphammer
Updated
August 25, 2005
by Contact:bascdean@lsu.edu
